Chii chinofanira kutariswa kana uchishandisa ndiro dzembudzi?

A petri ndiro ishandare yemabharabheti yakashandiswa kukura microorganisms kana tsika tsika. Iyo ine yeflat flat-yakavezwa yakavezwa uye chivharo. Zvinyorwa zvePetri ndiro zvakakamurwa kuita mapoka maviri, kunyanya mapurasitiki negirazi. Zvishongedzo zvemukati, tsika dzezvinyorwa zvecrobial, uye tsika dzakabatanidzwa dzegirazi dzemasero emhuka anowanzoshandiswawo. Iyo plastiki yakagadzirwa nePolyethylene uye yakakodzera kurambidzwa kwekutaura, kuiswa uye kuzviparadzanisa mabhakitiriya uye zvakare kune tsika dzetsika.

Yakatangira yakagadzirirwa muna 1887 neBacteriologist Julius Richard Petri (1852-1921) Pasi peGerman Biologist Robert Kober, saka inozivikanwawo se "goundo". Li's Petri dhishi ". Iyo petri ndiro haina kubatwa nehanya kana ichicheneswa uye nekuchenesa neyekuchengetera nzvimbo yakashandiswa uye ichichengetedza munzvimbo yakachengeteka uye yakasimba kuti udzivise kukuvara nekuputsa.

1. Kushambidza kwePetri ndiro

a) Kukuvadzwa: nyowani kana yakashandiswa girazi inofanira kunyudzwa mumvura kutanga kupfava uye inovhara izvo zvinongedzo. New Glassware Inongoda Kuchinjika Nemvura yeTap usati washandiswa, uye wobva wanyudzwa mu5% hydrochloric acid over hard; yakashandiswa girazi rinowanzove rine ruropliin yakawanda uye mafuta akasungirirwa nazvo, izvo zvisiri nyore kushambidza mushure mekuoma. Naizvozvo, inofanira kunge yakanyudzwa mumvura yakachena uye yakacheneswa nekukurumidza mushure mekushandiswa.

b) Kuongorora: Isa girazi rakanyoroveswa mumvura yekubvisa, uye inokandira kakawanda nejira nyoro. Usasiye nzvimbo yakafa uye dzivisa kukuvadza iyo yekupedzisa yekupedzisa yekuisa. Geza uye pukuta iyo yakacheneswa girazi rekutora.

c) Kutora: Kutora ndiko kudzikisira zvinhu zvinotaurwa pamusoro apa mumhinduro yekuchenesa, zvakare inozivikanwa semhinduro yewidhi, kubvisa zvinhu zvinogona kuramba zviri pamusoro pechinhu chakasimba nekuda kweiyo acid. Kutora hakufanirwe kunge kusingasviki maawa matanhatu, kazhinji kazhinji usiku kana kupfuura. Chenjera nemidziyo.

d) Rinse: Tageware inofanira kusurukirwa zvizere nemvura mushure mekuputika uye kupaza. Kunyangwe iyo midziyo yakacheneswa mushure mekutora zvakananga kubata kubudirira kana kutadza kwetsika tsika. Ruoko rwekugeza kwemidziyo. Imwe neimwe midziyo inofanira kudzokorora "mvura-inodururwa" kanokwana kaviri, uye pakupedzisira ndakashambidzwa ka3, nemvura yakapetwa kaviri, yakaoma kana yakaoma kana yakaoma kana kuomeswa uye yakarongedzwa.

E) Stetilization: inoraswa plastiki petri ndiro dzinowanzo shallised neirladiation kana makemikari sterilization kana vachisiya fekitori.

2. Kugadziriswa kwePetri ndiro

a) Zvinoenderana nekushandiswa kwakasiyana kweiyo petri ndiro, inogona kukamurwa kuita sero tsika uye bhata tsika ndiro.

b) Maererano nezvinhu zvakasiyana zvekugadzira, zvakakamurwa peturu petri ndiro uye girazi petri ndiro, asi zvose zvinotumirwa petri ndiro uye zvinoraswa ndiro petri ndiro dzinogadzirwa nepurasitiki zvinhu.

c) Zvinoenderana nehukuru hwakasiyana, zvinogona kugoverwa mumidziyo yepetri ine madhiri e 35mm, 60mm, 90mm uye 150mm uye 150mm.

d) Maererano nezvikamu zvakasiyana, zvinogona kukamurwa kuita 2-akapatsanurwa petri ndiro, 3-akapatsanurwa petri ndiro, nezvimwe.

E) Izvo zvinyorwa zvePetri ndiro zvakakamurwa kuita mapoka maviri, kunyanya mapurasitiki uye girazi, uye girazi rekudyara uye tsika yemuchero yetsika. Vapurasitiki vanogona kugadzirwa ne polyethyle, uye pane vakashandisa-kushandiswa-kushandiswa, izvo zvakakodzera kuti uwedzere kuyananiswa, kuyerera, uye kuzviparadzanisa mabhakitiriya, uye kunogona kushandiswa kurimwa kwezvakawanda zvekudyara.

3. Nyaya dzinoda kutarisisa mukushandiswa kwetsika dzetsika

a) Mushure mekuchenesa uye nekuwirwa nekushandisa usati washandiswa, kunyangwe iyo petri ndiro yakachena kana isina pesvedzero huru pabasa, iyo inogona kukanganisa iyo pH yeiyo svikiro. Kana paine mamwe makemikari, ichavimbisa kukura kwehutachiona.

b) Iyo ichangobva kutengwa petri ndiro dzinofanira kusukwa nemvura inopisa kutanga, uye yakabva yanyudzwa mune hydrochloric mhinduro ye1% kana 2% kwemaawa akati wandei kuti ubvise zvinhu zvakasununguka zveAlkaline, uye wobva wasukwa kaviri nemvura yakadzika.

c) Kana iwe uchida kurima mabhakitiriya, woshandisa yakakwirira-yekumanikidza Steam (kazhinji 6.8 * 10 kusvika ku12 ° C kwemaminetsi makumi matatu, kana yakaoma tembiricha, kana kuomesa kupisa kupisa , ndiko kuti, peta petri dish isa muvheni uye chengetedza tembiricha pane anenge zana nemashanu c kwemaawa maviri kuti uuraye masero ehutachiona.

d) chete yakasvibiswa petri ndiro dzinogona kushandiswa kuongororwa uye tsika.