Kuisa chisimbiso uye kusvibiswa nyaya muhuwandu hwetsika tsika

Kuisa uye kubata kweiyo yakawanda-yakanaka cell tsika mahwendefa:

Iyo cell yeClatelate ndiro inotevera iyo musimboti wekusimbisa kwakasimba kana uchiita sero mashandiro. Mashandiro ese anofanirwa kuve akaenzana uye musainzi, uye haazokonzeri kuwedzera kukanganisa kwekukura kwesero. Chinetso chakajairika ndechekuti ungaita sei kuti uve nechokwadi chemasero mushure mekuwedzera sampula uye kuderedza maitiro ekuchinja svikiro pamasero emasero.

Bvunza:
Iwo mahwendefa egumi nemakumi matatu nemashanu uye makumi maviri nemashanu emvura kana midziyo yepetri yakasununguka kwazvo, iri nyore kune ventilation, asi mabhakitiriya, akaumbwa uye mamwe magetsi anotsvedza mukati?

Mhinduro:
Iyo lid yakasununguka kwazvo, inova yeiyo hafu-yakavhurika tsika, uye chinangwa cheiyi yekuvhara (chokwadi, ndeyekuita iyo com pakati).
Zvese zvine zvakanakira uye zvakashata, izvo zvechokwadi zvinowedzera mukana wekusvibiswa. Uye zvakare, izvi zvinokonzeresa kuti mvura iri mundiro yekuputika, iyo inozivikanwa yekuita danho rezvinodhaka. Naizvozvo, matanho maviri anotevera anodikanwa: A. Mhepo iri muCompor inofanira kunge yakachena (yakajairwa Ultraviolet mwenje, doro richitsvuka, uye iyo incubator inofanira kuvhurwa uye yakavharwa zvishoma sezvinobvira) b. Iko kuomarara mune iyo incubator kunofanirwa kugara kuchengetwa pa100% (iyo yakanyura nemvura yakasvibirira yakaiswa mune incubator).
Senge petri ndiro, iriwo mudziyo une chivharo chepamusoro-pasi, uye hazvizosvibiswa. Kunyanya nekuda kwekumanikidzwa kwekutyaira kwakagadzirwa ne "l" kumucheto kwechifukidzo, kune guruva rakabatanidzwa kuguruva, uye guruva rinotakurwa nemhepo harigone kupfuura nemucheto wechifukidziro chinoburitsa zvakashata. Mhedzisiro yekuvira iri kuburikidza nekusiyana kwemhepo, uye hapana akaomeswa anozogadzirwa, saka zvichangofema uye kwete mabhakitiriya akapinda.

Bvunza:
Uchishandisa ndiro 24-zvakanaka, pane mashandiro mune mamwe matsime (mune iyo Ultra-yakachena bhenji), uye pane masero kuti aenzane mune matsime. Ndiri kunetseka kuti izvi zvichaita kusvibiswa. Ini handizive zvekuteerera?

Mhinduro:
Kana iko kuvhiya kwacho kwacho kwakagadziriswa muBench-akachena mubhenji, zvinofanirwa kunge zvakanaka. Ini ndinofunga iwe unogona kushandisa zvizere chifukidzo chetsika yetsika, edza kufumura chete maburi kuti ubatwe, uye kufukidza mamwe maburi ane michero.
Fungai kusanzwisisika usati washandisa uye kushandisa zvizere maburi ese; Kana iwe uchingoda kushandisa maburi mashoma, unogona kushandisa rimwe chete divi, uye kufukidza zororo nebhuku. Ini ndajaira kushandisa gomba rekurudyi kutanga (zvakanakira kuwedzera samples kuruoko rwerudyi).

Kana uchishandisa girazi shoma masiraidhi kusimudza rimwe divi rebhodhi, usavhura chivharo zvachose, kazhinji hapana dambudziko.
Kusarudzika Cell Kugoverwa uye Mhinduro

Bvunza:
Masero anounganidzwa pabhasikoro retsika, uye masero anogara achiungana muparutivi, maitiro ekuita nayo?

Mhinduro:
Ndingabvunze kuti masero ako akasanganiswa sei? Iyo pombetting kana kudedera ndiro yetsika? Kana iri yekupedzisira, uye kana ikazungunuswa mudenderedzwa, zvinoita kuti masero anokandwa kune chikamu chePeripheral nekuda kwesangano reCentrifugal, zvichikonzera masero mashoma pakati. Anopfuura mavhiki mana!
Iyi inzira yakanaka: usati wachirima ndiro yembeu, isa iyo tsika yetsika muIncubator kwemaawa mashoma ekuzadzika uye obva atora kunze. Simba rinofanira kuve rakareruka kana richidyara masero. Kuwedzera zvishoma nezvishoma kunobvumira iyo sero kumiswa kuti ifambe mumatsime eplate, uye masero ezvakawedzera anonyanya kukura. Rangarira kusazombotambudza nemuridzi, kana masero ako anofamba pamwe chete sezvamakataura.
Iyo diki iyo gomba rairo retsika yemapepa ezvetsika, zvakanyatsojeka chiitiko ichi. Ichi chiitiko hachina kuratidziriki kwemakumi maviri nemapfumbamwe, nekuti mvura inonamira kumadziro kuitira kuti sosi yetsika iri patsime haina kuumba mvura yemvura, asi girazi rakakwirira, segirazi rakamanikana. Nekudaro, kana uchishandisa idzi marudzi maviri emhando dze orifeti, hazviiti kuwedzera huwandu hwenzvimbo yetsika nekuda kwekuda kwekuwedzera kupindira, kuitira kuti masero aonekwe "muunganidzwa wetsika" pamwe chete nemhedzisiro yetsika. Zvinoenderana nezvinoda zviratidzo zvaunoda kuti ucherechedze. Kana iri mt
Paunenge uchiraya masero, teerera pipetting zvakanyanyisa kudzivirira cell clamp, uye huwandu hwemhedzisiro yetsika mutsime hunofanira kunge hwakaringana. Kazhinji, wedzera yakakwana tsika yekugadzirisa kana ichishungurudza, uye shandura mhinduro kamwechete kana uchiwedzera kupindira. Huwandu hwetsika mhinduro yakawedzerwa kumhinduro yekupindira yakaenzana nehuwandu hwetsika mhinduro panguva yekunyora, mune ino nyaya ye "chikamu che" mupendero wakagadziriswa "kuti iwe ugone kuraira.

Bvunza:
Zvandaita ndidzo dzaive dzidziso yekugadzira. Zvakanaka kuparadzira maseru muhari yunifomu. Mazhinji emakomba akanaka apo hutachiona huchikanganiswa, uye vamwe vacho havana kufanana. Kune misiyano mikuru muboka remasero. Ndinoda kukubvunza iwe nzira yaunoshandisa kana uchiwedzera masero?

Mhinduro:
Iyo inonyanya kukosha pipette masero mune imwechete-cell kumiswa mushure mekugeza! Ita shuwa kuti huwandu hwemasero mune imwe neimwe tsime inowirirana kana ikapatsanura ndiro!
Ehe, pane zvakare kugadzirisa zvinhu. Iko kufanana pakati petsime kunokoshawo kukosha. Semuenzaniso, paunenge uchiwedzera mushonga, sanganisa mushonga mushure mekudhira kuti uve nechokwadi chekuti kusunganidzwa kwetsime rimwe nerimwe kunoenderana!
Uyezve, kana uchiwedzera masero uye zvinodhaka, boka rekuyedza uye boka rekutonga rinofanira kuwedzerwa mukudzivisa musiyano wekuwedzera kwesero uye zvinodhaka zvakakonzerwa nekutevedzana kwekuwedzera samples.

Bvunza:
Kurova kwatove kwatove kwatove, asi mapuranga, matombo matanhatu, uye maburi makumi maviri nematatu anogara asina kufanana, uye zvishoma zvakanyanyisa pakati. Zvakare, zviri pachena kuti kuverenga kunoitwa, uye mushure mezuva kana maviri, density yegomba rega rega rakasiyana, rinozokanganisa kuwanikwa. Pane nzira yakanaka here?

Mhinduro:
Kana iwe ukashandisa pasteur pipette, usywe zvakanyanya nguva imwe neimwe, nekuti masero ari muPipette achanyura, kazhinji huwandu hwemasero mumakomba mashoma emaburi makuru, uye kumiswa kwemasero kunowanzove yunifomu, uye mvura ichaita tevera iyo s-chimiro nzira.
Kana iwe ukashandisa pipette, matipi anofanirwa kuongororwa uye akabviswa kuti arege kukuvadza masero, kuitira kuti nguva imwe neimwe iyo mvura ichitorerwazve patsime. Iyo yakarongeka yekuwedzera imwe-kune-imwe gomba ine tip. Asi zvakare teerera kusanganisa kumiswa.

Kuti uise boka rakafanana, n n n hangove rakakura zvakakwana (unogona kuverenga).

Usazungunuke mushure mekurongedza, sekuzununguka kuchakonzera masero ekuunganidza akananga kuCentre. Zvakanaka kune plank kamwe.

Porous Cell Tsika Plate Medical yagara ichitarisira kudzora maitiro sehupenyu hweBhizinesi, uye tevera kudzvanywa kwekuenderera kwebhizinesi kukwikwidza. Iyo kambani inogara ichinamatira kumudzimu yekuteerera mitemo nemirairo, kuve nekuzvidzora, kuve neshanje, uye kuchengeta uye kuchengeta uye kuwedzera musika nemhando yepamusoro uye kushumirwa kwakanaka, uye kusangana ne zvinodiwa zvevatengi kusvika pakukura kukuru. . Win-Win nevatengi ndiyo chinangwa chedu chekuvandudza. Yongyue Zvekurapa! Wako waunovimba naye. Isu tinoda kushandira pamwe nemwoyo wose kuti vagadzire ramangwana riri nani.