TC Kubata kweCell Change Tsika Danho Rinokosha kwazvo kuti uve nechokwadi chakaringana, chakavimbika uye chakabudirira mhedzisiro mukati mechero tsika. Chinyorwa ichi chinotsanangura chinangwa, nzira, uye maitiro akanakisa eTC kunyengedza uye kukurukura kukosha kwayo kwekutsvaga kwetsika. 1. Chinangwa cheTC kugadzirisa TC kurapwa TC kurapwa kunogona kuita kuti masero atore zviri nani kuzasi kwetsika, uye anogona kusimudzira kukura kwesero uye kupatsanura njodzi yekufa kwevanhu nekuda kwekudonha kwevarombo nekuda kwekushambidzika kusarongeka nekuda kwekududzira zvisina kunaka. 2. TC kugadzirisa nzira Kune nzira dzakasiyana siyana dzeTC kurapwa, kusanganisira nzira dzemuviri uye nemakemikari. Pakati pavo, nzira yepanyama ndeyekudzikisa kusawirirana kwesimba kwetsika kuburikidza neyakagadzirirwa kushushikana kuendesa mumiririri kusimudzira kushamwaridzana. Nzira yemakemikari ndeye kushandisa zvigadzirwa zvakaita seSloxane kuti ubike pamusoro peiyo tsika yetsika kuti uvandudze mvura yemvura. 3. Yakakodzera kudzidzira yeTC kugadzirisa Paunenge uchiita TC kurapwa, pfungwa dzinotevera dzinofanira kucherechedzwa: 3.1 Kuchenesa: usati waisa petri ndiro kune tc kurapwa, inofanira kuve yakavimbiswa kuti nzvimbo yayo yakachena, isina chinhu uye yakaoma. Izvi zvinogona kuwanikwa nekushandisa organic solvent (senge ethanol kana methanol) uye marara eUV. 3.2 Nguva: TC yekugadzirisa nguva inofanira kudzorwa nenzira kwayo. Tsika dzakasiyana-siyana dzekudya uye mhando dzekurapa dzinogona kuda nguva dzakasiyana dzekurapa. Naizvozvo, usati waita kurapwa kweTC, iwe unofanirwa kureva mirayiridzo kana mabhuku anopiwa nemutengesi, uye kugadzirisa zvakakodzera zvinoenderana nezvinodiwa zvekuedza. 3.3 Mhando Yekutonga: Mhedzisiro yeTC kurapwa ichakanganisa iyo inotevera cell tsika mhinduro. Kuti uve nechokwadi chekuti Batch yega yega yePetri ndiro dzinogona kuenderana neTC kurapwa kweTC. 4. Kukosha kweTC kurapwa kweTECER KWENYU YEMAHARA TC kurapwa kwete kungovandudza kusaenzana kwesero kushamwaridzana uye kukura kwekukura, asi zvakare kwakaderedza kufa kwesero uye kusimudzira chikamu checende. Zvakanakira izvi zvinogona kuita basa rakakosha muyedzo dzakawanda dzekuedzwa kwetsika, senge cell proliferation, kuongorora kwezvinodhaka, uye kuongororwa kwechisimba, uye etc.
